Proteomic Analysis for Target Discovery

Proteins are key executors of life activities and are the main targets of current drug development. Evaluating drug efficacy from the perspective of proteomics is of great significance for the detection, validation, and optimization of drug targets, which not only promotes the research and development of innovative drugs, but also provides strong support for clinical applications. Comprehensive proteome extraction, digestion, peptide fractionation, and high-resolution mass spectrometry data acquisition of biological samples, combined with efficient database search, enable in-depth qualitative and quantitative analysis of thousands to tens of thousands of proteins in samples.

 

Target discovery based on differential proteomic analysis typically involves comparing differences in protein expression in cells or tissues before and after disease versus normal physiological states or drug treatment to identify effective drug action targets. At ChemPro Innovations, we provide you with a set of standardized sample handling protocols, qualitative and quantitative proteomics identification, and specialized data analysis strategies.

 

Proteome Quantitative Analysis Technology at ChemPro Innovations

1.Label-Free Quantitation (LFQ)

Accurately analyze the protein content in different samples by comparing the number of spectra or the area of ion peaks.

 

Advantages: No isotope labeling required, allowing for rapid comparison of differences in protein abundance between large numbers of samples.

 

Applications: Cell, tissue, blood, etc.

 

2.Reductive Dimethylation Labeling Quantification Technique (ReDi)

Quantitative protein comparison between double or triple samples is achieved by utilizing an isotope tag with a reaction with the amino groups of the peptide lysine side chain and the N-terminus.

 

Advantages: Cost-effective and highly versatile.

 

Applications: Cell, tissue, blood, etc.

 

3.Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)

Accurate quantification of the proteome between different samples by culturing cells using media containing stable isotopes.

 

Advantages: Reduced operational error and more precise quantification.

 

Application: Live cell samples.

 

4.Tandem Mass Spectrometry Labeling Quantification (TMT/iTRAQ)

Peptide labeling with TMT/iTRAQ reagents compares protein quantification in different samples by detecting the signal intensity of the reporter in the sample.

 

Advantages: Allows analysis of up to 16 samples with extremely accurate quantitative results.

 

Applications: Cell, tissue, blood, etc.

 

Service at ChemPro Innovations

1.Provide quantitative analysis of proteome-wide differential proteins for the study of proteomic changes caused by conditions such as disease, drug treatment, and environmental stimuli.

 

2.Perform whole-proteome quantitative analysis of subcellular structures (such as cell membranes, nuclei, mitochondria, etc.).

 

3.Discover biomarkers at the proteome-wide level.