Analysis of PROTAC Target Protein and Degradation Profiles

 

Analyzing the on-target and off-target effects of Proteolysis Targeting Chimeric (PROTAC) molecules at the entire proteome level is a pivotal aspect in the development of drugs within this category.

 

The core of PROTAC technology is to develop bifunctional molecules that can simultaneously bind to the target protein and recruit degrading enzymes. This dual action guides ubiquitinating enzymes to approach the target protein, thereby facilitating its ubiquitin-mediated degradation process. This versatile technology finds applications in the treatment of various diseases.

 

Workflow

1.Using PROTAC molecules to process different cell samples

 

2.Extract the whole proteome

 

3.Protein digestion

 

4.Multiplex quantitative labeling

 

5. Mass spectrometry detection

 

6. In-depth analysis of protein abundance differences between samples

 

Services at ChemPro Innovations

1.Degradation mapping of PROTACs at the whole proteome level (quantitative identification of proteomics based on MS).

 

2.Verification of Target Protein Degradation(targeted quantitative protein analysis/western blot).